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. 2020 May 12;10:589. doi: 10.3389/fonc.2020.00589

Figure 5.

Figure 5

The effect of high-mobility group AT-hook 1 (HMGA1) knockdown on autophagy and growth, invasion, and migration of bladder cancer (BC) cells. (A) Cell Counting Kit-8 (CCK-8) cell viability assay of T24 cells treated with siHMGA1. (B) The protein expression of HMGA1, poly (ADP-ribose) polymerase (PARP), p62, LC3, TP53INP1, p-ERK1/2, and ERK1/2 in T24 cells transfected with HMGA1 siRNA. (C) T24 cells were transfected with HMGA1 siRNA for 48 h and then stained with Cyto-ID Autophagy Detection Kit and LC3 antibody. Scale bar, 50 μm. (D) Cell clone numbers were counted when HMGA1 or ATG5 was silenced in T24. (E) The effect of HMGA1 or ATG5 knockdown on the invasive ability of T24 cells as detected by transwell invasion assay. Scale bar, 50 μm. (F) The effect of HMGA1 or ATG5 silencing on cell migration of T24 cells performed by wound healing assay. Scale bar, 100 μm. *p < 0.05, **p < 0.01 and ns, no statistical significance.