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. 2020 May 12;7:80. doi: 10.3389/fcvm.2020.00080

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Copyright © 2020 Amadeo, Barbuto, Bernava, Savini, Brioschi, Rizzi, Banfi, Polvani and Pesce.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular phenotype and dynamics inside recellularized pericardia. (A–C) Immunofluorescence staining showing expression of αSMA, PCNA, and YAP (all in green fluorescence) in conjunction with Vimentin (in red fluorescence) inside the recellularized pericardial tissue at day 3 and 21 of culturing. In each panel the lower micrographs show the magnification of the boxed insets in the higher photographs. (D) Quantification of total number of cells by DAPI-stained nuclei counting (upper bar graph), PCNA⁺ cells (middle bar graph) and cells with nuclear localized YAP (nYAP⁺ cells, low bar graph) at all the considered time points. Statistical analysis was performed with one-way ANOVA with Newman Keuls post-hoc; ^* indicates P < 0.05 (n = 3).