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. 2020 May 18;10:8137. doi: 10.1038/s41598-020-65035-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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scFv-pF and scFv-pC reduce the toxicity caused by α-syn seeds in SH-SY5Y cell model of PD. The viability of SH-SY5Y cells was evaluated using MTT assay (a) or LDH activity assay (b). 2 µM α-syn seeds were incubated with 0.25 µM Syn-F2, 0.25 µM scFv-pF, or 0.25 µM scFv-pC. After incubation, 10 µM α-syn monomers were then added in Opti-MEM for 48 hours prior to MTT addition. For MTT assay, the results are expressed as percentages of the average of the control (untreated cells). For LDH assay (b) 50 μl media was removed before the addition of MTT reagent and released LDH activity was measured. The absorbance was measured and percentage of the viable cells or LDH activity was plotted (average of 3 wells ± standard deviation). Statistical analysis was performed using one-way ANOVA with Dunnet’s multiple comparison test. (***p < 0.001; **p < 0.01; *p < 0.05).