Fig. 2. Results of the general linear model that includes the social responsiveness scale (SRS-2) as the dependent variable and levels of methylation at CpG 5,6 (b.p. −1121, −1119) as well as age as predictors.

The X axis represents the predicted estimated value of both predictors in relation to the dependent variable. a Positive correlation between methylation at CpG 5,6 (b.p. −1121, −1119) and total scores of SRS-2 (χ² = 5.25, P < 0.05). b Negative correlation between methylation at CpG 16 (b.p. −989) and ADI-R total scores (χ² = 4.92; P < 0.05). c Correlations between methylation at CpG 16 (b.p. −989) and ADI-R subcomponents (χ² = 4.71; P < 0.05). SI social interaction, LC language and communication, RRB restricted and repetitive behavior. Dashed lines denote the 95% confidence interval.