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. 2020 May 7;23(6):101139. doi: 10.1016/j.isci.2020.101139

Figure 5.

Figure 5

Development and Validation of the Notch1-based N100 Y-T Biosensor

(A) Schematic representation of the sequence of N100 Y-T biosensor. The APP C99 in the C99 Y-T (80 a.a.) biosensor was replaced with Notch1 N100.

(B) Combined full-length N100 Y-T and NICD Y-T bands are detected with the HA antibody in Western blotting using NuPAGE 4%–12% Bis-Tris Protein gels. The NICD-specific cleaved Notch1 Val1744 antibody reveals cleaved NICD Y-T band only, which is diminished by the treatment with DAPT.

(C) The overall 531(Y)/489(T) ratio in DAPT-treated CHO cells expressing the N100 Y-T biosensor is significantly higher than that in vehicle control (n = 78–81 cells). Mean ± SD, ∗∗∗p < 0.001, Student's t test.