Figure 6.

α-SYN regulated the transcription activity of Nurr1 via NF-κB. (A) The schematic diagram of NF-κB binding site luciferase plasmid. Dual-Glo luciferase assay determined the transcription factor activity of NF-κB in control cells (vector), α-SYN^WT (WT) and α-SYN^A53T (A53T) transfected groups (B). ChIP-real-time PCR assay examined NF-κB or α-SYN occupancy of Nurr1 promoter in α-SYN overexpressed cells (C). The amplified fragments were detected by 2% agarose gel (D). After cytoplasm nucleus extraction, the nucleus and cytoplasm (E) protein expression of NF-κB was detected by Western blot. Data were expressed as mean ± SEM. **p < 0.01 vs. control cells, n = 3.