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. 2020 May 12;13:75. doi: 10.3389/fnmol.2020.00075

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Copyright © 2020 Tsuneoka and Funato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-dependent increase in fluorescence of in situ HCR using short hairpin DNAs. Penk mRNA expression in the mouse striatum was detected by Alexa647-conjugated hairpin DNA (#S41) and corresponding probe sets. Background signals were not subtracted. (A–C) Sections were hybridized with split-initiator probes followed by HCR amplification. (D–F) Sections were treated without probes, which was followed by HCR amplification. (G–I) Sections were hybridized with probes followed by only H1 hairpin DNA hybridization. (A,D,G) Incubation for 45 min with hairpin DNAs. (B,E,H) Incubation for 2 h with hairpin DNAs. (C,F,I) Overnight incubation with hairpin DNAs. Scale bars: 200 μm.