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. 2020 May 12;13:75. doi: 10.3389/fnmol.2020.00075

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Copyright © 2020 Tsuneoka and Funato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Redundant detection of Moxd1 mRNA using different probes. (A) In situ HCR using different probe-hairpin sets designed for different regions of the Moxd1 mRNAs. HCR for 45 min detected Moxd1 mRNAs in the bed nucleus of the stria terminalis. Five probe pairs were used for each channel. Fluorescent signals in Ch1 and Ch2 were detected using ATTO550-conjugated hairpin DNA (#S41) and Alexa647-conjugated hairpin DNA (#S25), respectively. (B) Representative photomicrograph showing subcellular resolution using the same probe-hairpin sets as in panel (A). Dotted circles indicate granules detected in both channels, and arrows indicate granules detected in one of the two channels. Scale bars: 20 μm (A) and 2 μm (B).