TABLE 1.

Primer design.

Gene	RefSeq (Bos taurus)	Forward primer	Reverse primer	Tm (°C)	Amplicon size (bp)
RPL19	NM_001040516.1	GAAAGGCAGGCATATGGGTA	TCATCCTCCTCATCCAGGTT	60	86
PPIA	NM_178320.2	CATACAGGTCCTGGCATCTTGTCC	CACGTGCTTGCCATCCAACC	60	108
CALM1	NM_001242572.1	GGATGGCAACGGGTACATCA	CTCCTCGTCCGTCAGCTTC	60	79
CASP3	NM_001077840.1	ACCAACGGACCCGTCAATTT	CCTCGGCAGGCCTGAATAAT	60	107
CITED1	NM_174518.1	TCACCTCCCACCAATTTATCCAA	TTGGCATTCTCCTTCACAGGT	60	110
DLD	NM_001206170.2	CGATGGCAGCACTCAAGTTA	CCTTGTTTTTGAAGGATACGTTG	60	306
FURIN	XM_024981598.1	GTTCGGCAACGTGCCCTG	TTCTTATTGGCCTCCAGGGTGAG	60	195
GSTM1	XM_010803234.3	GGACTTTCCCAATTTGCCCTAC	GCAATGTAGCGAAGGATGGC	60	78
HNRNPDL	XR_235028.4	GTGGCTATGGCGGCTATGAT	TGTTGGCCACTGTAGTCTGC	60	85
IL6	NM_173923.2	GCGCATGGTCGACAAAATCT	AAATCGCCTGATTGAACCCAGA	60	158
JSP1	XM_024983412.1	TTCCTCACCATGGGCATCATTG	ATCGTTATTCTGTTCCCGGCTG	60	172
PTGS2	NM_174445.2	CTGATGTTTGCATTCTTTGCCC	CTTAAGTCCACCCCATGGTTCT	60	107
TGFB3	NM_001101183.1	ACATAGCCAAGCAGCGGTAT	CCTAAGTTGGATTCTCTCCGCA	60	124

RefSeq corresponds to Gene NCBI accession number. PCR conditions: melting temperature in °C (Tm) and amplicon size in bp.