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. 2020 May 12;11:890. doi: 10.3389/fmicb.2020.00890

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Copyright © 2020 van den Berg van Saparoea, Houben, Kuijl, Luirink and Jong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spy ligation to the Hbp display platform containing an internal SpyTag. OMVs containing the Hbp display platform with a SpyTag at different positions (domain 1, 2, or 4) between either five amino acid long linkers (5/5) or ten amino acid long linkers (10/10) were incubated with SpC-MBP (M) or with SpC-TrxA (T). Protein ligation was analyzed by SDS-PAGE with Coomassie staining. Identical amounts of OMV material (OD₆₆₀ units) were loaded in each lane. Below the gel image lane numbers are indicated.