TABLE 3.
Spy- and Snoop-coupling efficiencies to HbpD displayed on OMVs.
| Displayed HbpD variant | Ligation product | |||||
| SnC-… (to d1) | SpC-… (to d2/d4) | SnC-… + SpC-… | ||||
| HbpD-(d1)SnT-(d2)SpT (112) | TrxA (139)∗ | 88%# | MBP (168) | 96% | TrxA + MBP (193) | 71% |
| HbpD-(d1)SnT-(d2)SpT | (ND)∧ | TrxA (137) | 100% | (ND) | ||
| HbpD-(d1)SnT-(d4)SpT (120) | TrxA (147) | 92% | MBP (173) | 98% | TrxA + MBP (201) | 84% |
| HbpD-(d1)SnT-(d4)SpT | (ND) | TrxA (145) | 100% | (ND) | ||
| SnT-… (to d1) | SpC-… (to d2/4) | SnT-… + SpC-… | ||||
| HbpD-(d1)SnC-(d2)SpT (123) | TrxA (139) | 69% | MBP (176) | 99% | TrxA + MBP (192) | 43% |
| HbpD-(d1)SnC-(d2)SpT | MBP (168) | 26% | TrxA (148) | 99% | MBP + TrxA (193) | 24% |
| HbpD-(d1)SnC-(d4)SpT (131) | TrxA (147) | 55% | MBP (184) | 99% | TrxA + MBP (200) | 28%& |
| HbpD-(d1)SnC-(d4)SpT | MBP (176) | 25% | TrxA (156) | 93% | MBP + TrxA (201) | 26% |
∗Protein molecular mass in kDa is given between brackets. #Percentage of Hbp ligated with SnC-, SpC-, and/or SnT-fusion protein(s). ∧SnC-MBP was rather prone to aggregation and not further analyzed in coupling experiments. &Estimate because the single and double adducts have similar mobility in SDS-PAGE.