Expression of dmrt3a and related genes in dmrt3a47aa and dmrt3aWT. A, Relative expression of dmrt3a mRNA from 1 to 5 dpf. B, Schematic of a 3 dpf larva where relevant structures are labeled in purple [i.e., hindbrain (HB), spinal cord (SC), olfactory bulb (OB), and kidney (K)]. C–J, mRNA pattern expression at 3 dpf. Top and lateral views: images of dmrt3a47aa and dmrt3aWT represent the average expression of 5 larvae. Merged color images below show the expression from dmrt3aWT in green and from dmrt3a47aa in magenta. Top view: C, dmrt3a expression; D, dmrt1a expression; E, dmrt2a expression; F, wt1a expression. Lateral view: G, dmrt3a expression; H, dmrt1a expression; I, dmrt2a expression; J, wt1a expression. Abbreviations highlight relevant labeling. This figure is extended in Extended Data Figure 5-1.