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. 2020 May 12;11:891. doi: 10.3389/fimmu.2020.00891

Figure 1.

Figure 1

IL-1 inhibition in susceptible mice reduces inflammation with no effect on lung bacterial burden. Wild type C57BL/6 and Nos2−/− mice were infected with Mtb Erdman for 2 weeks and treated with anti-IL-1R1 (αIL-1R1) for the subsequent 2 weeks. (A) Lung neutrophil/PMN (CD45+ CD11b+ Ly-6C− CD3e−Ly-6G+), T-cell (CD45+ Ly6C−LyG−CD3e+), and Monocyte/macrophage (CD45+ CD11b+ Ly-6G− CD3e− Ly6C+) recruitment to the lung among different treatment groups were quantified by flow cytometry. (B) Weight loss and (C) CFU in lung and (D) spleen are shown. Data shown (mean ± SD) are representative of two independent experiments. One-way ANOVA with Sidak's multiple comparisons-test was used. N = 4 mice per treatment cohorts. (E) Histopathology analysis of a single lung lobe from WT or Nos2−/− mice was performed by Hematoxylin-eosin (H&E) staining and (F) Lesion size was quantified by analyzing the histopathological images from each mouse lung among treatment groups (n = 4). Data shown are expressed as Mean ± SD. One-way ANOVA with Tukey's multiple comparison test was applied to calculate the p-value.