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. 2020 May 12;11:891. doi: 10.3389/fimmu.2020.00891

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Copyright © 2020 Winchell, Mishra, Phuah, Saqib, Nelson, Maiello, Causgrove, Ameel, Stein, Borish, White, Klein, Zimmerman, Dartois, Lin, Sassetti and Flynn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Linezolid-associated bone marrow suppression was not observed in macaques. Bone marrow was acquired from the sternum of all macaques at necropsy after 4 weeks LZD or LZD+IL-1Rn. A portion was used for flow cytometric analysis of erythroid progenitor populations and the rest for histopathology analysis. (A) A schematic of erythropoiesis indicating the stage of differentiation and corresponding expression levels of phenotyping markers (CD235a and CD71) and progenitor ratios (1:2:4:8). Included is the gating strategy to identify progenitor populations and an example of MitoTracker staining. (B) The ProE:EryC ratio (left) of each animal, including untreated TB only macaques (black) from other ongoing unpublished studies. A single uninfected animal (open circle) is shown as reference, the dotted line indicates the homeostatic ratio of 1:8. MitoTracker MFI (right) is shown for each macaque per treatment group. Kruskal-Wallis test was performed to compare the three groups (excluding uninfected macaque) and p-values are shown in the graphs. (C) Representative H&E images from sternal bone marrow acquired at necropsy, 20x (left) and 40x (right) are shown.