Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 12;11:469. doi: 10.3389/fgene.2020.00469

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Zhou, Benbow, Brennan, Arunachalam, Karki, Mullins, Feechan, Burke and Doohan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Validating the cellular secretion of wheat small secreted proteins (TaSSPs) secretion using a yeast expression system. The expression of the TaSSPs with a positive secretion signal will result in the secretion of the SUC2 protein allowing the growth of yeast on sucrose-containing media. The TaSPP6 and TaSSP7 proteins with secretion signal removed cannot grow on media. The yeast strain transformed with SUC2^FullLength (with signal peptide) gene was used as a positive control while the strain transformed ΔSP:SUC2²²^–⁵¹¹ (without signal peptide) gene was used as a negative control. Yeast was spotted onto the media in a serial dilution from an initial OD₆₀₀ of 1.0 to 0.1, 0.01, and 0.001, respectively.