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. 2020 May 12;11:469. doi: 10.3389/fgene.2020.00469

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Copyright © 2020 Zhou, Benbow, Brennan, Arunachalam, Karki, Mullins, Feechan, Burke and Doohan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virus-induced gene silencing (VIGS) of TaSSPs increases susceptibility to STB disease in the STB-resistant cv. Stigg. (A) Representative photos of STB-treated leaves of plants that were inoculated with either the empty vector (BSMV:00) or a silencing vector. (B) Leaf area showing necrosis was significantly higher (^∗) in plants with silenced TaSSP6 and TaSSP7 that were treated with Zymoseptoria tritici. Both silencing constructs of both TaSSP genes had the same effect on the disease response. Silencing of TaSSP6 (C) and TaSSP7 (D) was confirmed via qRT-PCR; both constructs of both TaSSP genes significantly (^∗P < 0.05) reduced transcript abundance of the genes in plants treated with Z. tritici.