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. 2020 May 12;11:469. doi: 10.3389/fgene.2020.00469

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Copyright © 2020 Zhou, Benbow, Brennan, Arunachalam, Karki, Mullins, Feechan, Burke and Doohan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TaSSP proteins interact with ZtSSPs in yeast. Matchmaker Gold yeast strains carrying the bait vector pGADT7 containing TaSSPs were transformed with the prey vector pGBKT7 containing ZtSSPs. Strains were spotted on synthetic defined (SD) selective media (lacking leucine, tryptophan, histidine, and adenine, -LTHA) and incubated at 30°C for 3 days. Wheat protein TaTRG7 (in blue) was used as a negative control for the TaSSPs and Zt18 was used as a negative control for Z. tritici SSPs (in red). Empty vector controls are shown in the right-hand panel.