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. 2020 May 12;14:97. doi: 10.3389/fncel.2020.00097

Figure 5.

Figure 5

Inhibition of the MALAT1/miR-224-5p/NLRP3 axis reduced inflammation caused by exposure to IH and HG. (A) Sequence alignment between miR-224-5p and the 3′-untranslated region (UTR) of NLRP3. Complementary bases between the sequences are shown in red. The sequence of the mutant NLRP3 construct is also shown. (B) Firefly luciferase assay of BV2 cells co-transfected with NLRP3 3′-UTR-WT or NLRP3 3′-UTR-Mut and miR-224-5p mimic or miR-NC. Data are presented as the mean ± SD from six separate experiments. **p < 0.01. (C,D) After the transfection of mimics and inhibitor, the mRNA expression and protein expression of NLRP3 was determined by qRT-PCR (C) and western blotting (D). (E,F) NLRP3, caspase 1, TNF-α and IL-1β expression levels in different groups of BV2 cells were detected by qRT-PCR (E) and western blotting (F). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the NC group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the HG+IH+miR-224m group.