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. 2020 Apr 23;23:21–28. doi: 10.1016/j.jot.2020.03.007

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Migration of UCMSCs to SFCs migration by CHO was enhanced after MIA induction and UCMSCs at early stage of OA. (A) One week after MIA treatment, we could see enriched elongated cells in the injured uneven cartilage surface (lower panel, arrow), whereas elongated SFCs evenly distributed on normal articular cartilage surface (upper panel). (Scale bars, 100 μm.) (B) Crystal violet staining of transwell assay revealed obviously more migrated SFCs by serum-starved CHOs in the lower chamber compared with vehicle-treated CHOs. (C) Transwell assay showed that UCMSCs-CM substantially promote SFCs migration compared with vehicle (DMEM). (Scale bars, 50 μm.) (D) Quantification of B. ∗p ＜ 0.05. (E) Quantification of C and quantification data of E. ∗∗p < 0.01. Results are representative of at least three independent experiments and expressed as mean ± standard deviation. CHO = chondrocytes; MIA = monosodium iodoacetate; OA = osteoarthritis; SFCs = superficial layer cells; UCMSCs = umbilical cord–derived mesenchymal stem cells; UCMSCs-CM = UCMSCs-conditioned media.