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. 2020 Apr 25;39:101005. doi: 10.1016/j.molmet.2020.101005

Figure 1.

Figure 1

Autophagy is induced by PKA signaling in adipocytes in vitro. (A–B) Immunoblot analysis of LC3 and HSL in adipocytes differentiated from C3H10T1/2 cells. Earle's Balanced Salt Solution (EBSS) was used for nutrient deprivation. Cells were treated with 1 mM 8Br-cAMP for 4 h (n = 6 per condition, mean ± SEM, ∗P < 0.05, ∗∗∗P < 0.001). Cells were pretreated with chloroquine (CQ: 50 μM) or 3-methyl adenine (3 MA: 5 mM) for 30 min before 8Br-cAMP or vehicle treatment. (C) Representative images of adipocytes differentiated from C3H10T1/2 cells treated with 8Br-cAMP for 4 h and stained for LC3. LipidTox and DAPI were used for neutral lipid and nucleus staining, respectively.