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Autophagy is induced by beta-adrenergic stimulation in adipose tissue in vivo. (A) Immunoblot analysis of LC3, SQSTM1, and HSL in BAT and gWAT of mice. (B) qPCR analysis of Sqstm1 in BAT and gWAT. (C) Immunoblot analysis of LC3 and HSL in BAT of mice treated with CL316243 for 4 h and controls. (D) Immunoblot analysis of LC3, SQSTM1, tyrosine hydroxylase (TH), and HSL of denervated BAT and sham-surgery controls (n = 8 per condition, mean ± SEM, ∗∗∗P < 0.001).
