Figure 8.

Beclin1 KO in cultured brown adipocytes induces cell death and impairs autophagy-related and mitochondrial protein expression. Cell death analysis of adipocytes differentiated from precursors obtained from BAT of Beclin1^adipoqKO mice and WT controls. Adipocytes differentiated from the precursor cells were treated with 4-hydroxyl-tamoxifen (1 μM) to delete Beclin1. (A) LIVE/DEAD staining by CaleinAM (green) and EthD-1 (red). (B) FACS analysis of Annexin V. (C–D) Immunoblot analysis of autophagy-related and mitochondrial proteins (n = 6 per condition, mean ± SEM, t-test, ∗∗P < 0.01, ∗∗∗P < 0.001).