Figure 4.

Technical considerations of the pressure-clamped single-fiber recordings. (a) Two sample traces on the top show RA impulses recorded from a single whisker afferent nerve fiber at the beginning (left) and 1 h later (right); the RA impulses were evoked by a 38-µm displacement step applied onto the whisker hair follicle in both recordings. The two images on the bottom show the nerve end that was pressure-clamped within the recording electrode at the beginning (left) and 1 h later (right). (b) Sample trace on the top left showing SA2 impulses recorded from a single whisker afferent nerve fiber which was aspirated too deep into the recording electrode (image on the bottom left). The amplitudes of the impulses were increased (top right) after reducing the internal pressure in the recording electrode to readjust the fiber fitting in the recording electrode (bottom right). The SA2 impulses were evoked by a 38-µm displacement step applied onto the whisker hair follicle in both recordings.