Figure 1.
Antibacterial assays of pure compounds against P. aeruginosa. Briefly, 50 µg/mL of each compound was incubated with 1 × 106 bacteria at 37 °C for 2 h. Next, cultures were serially diluted and plated onto agar plates. Following this incubation, viable bacterial colonies were counted and c.f.u. was recorded. (a) Bactericidal activities of curcamenol, L-homotyrosine and docosanedioic acid against P. aeruginosa. (b) Representative effects of compounds on P. aeruginosa. The data are expressed as the mean ±SE of several independent experiments performed in duplicate. P-values were determined by student’s T-test where (*) represents P < 0.05 using GraphPad Prism 8.0.2.