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. 2020 Mar 30;9(4):148. doi: 10.3390/antibiotics9040148

Figure 7.

Figure 7

Enzyme activity of the recombinant MurNac-LAA domain. Enzyme activity was demonstrated by measuring the time-dependent turbidity changes in a suspension of S. epidermidis (ATCC 12228) cells. Changes in turbidity was measured spectrophotometrically at 600 nm at regular time intervals and at a fixed temperature (25 °C), using extract from recombinant E. coli BL21(DE3) cells (●), compared to a negative control using untransformed E. coli BL21(DE3) cells (○).