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. 2020 Apr 11;8(4):86. doi: 10.3390/biomedicines8040086

Figure 3.

Figure 3

Effect of tussilagone on NF-κB and Nrf2 activities in the colon tissues. (a) The protein samples in cytosol or nuclear fraction from the colon tissues were separated by SDS-PAGE. NF-κB and Nrf2 were detected by Western blot and visualized by chemiluminescence. Photos are representative images. (b) The quantitative data were normalized by internal control (β-actin for cytosol; lamin-B for nuclear fraction) and further expressed as folds, presented as the comparison with the amount relative to the AOM/DSS-treated mice. (c) The immunohistochemical analysis of nuclear NF-κB expression in the colon tissues (upper, ×100 magnification; lower, ×400 magnification; bar = 100 µm). (d) The percentage of nuclear NF-κB positive cells in colon tissues was calculated as described in Methods. (e) The immunohistochemical analysis of nuclear Nrf2 expression in the colon tissues (upper, ×100 magnification; lower, ×400 magnification; bar = 100 µm). (f) The percentage of the nuclear Nrf2 positive cells in colon tissues was calculated as described in Methods. Results are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, compared to the AOM/DSS-treated mice.