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Horseradish peroxidase (HRP)-cortisol-coated microchip dipped in a 3,3′,5,5′-tetramethylbenzidine (TMB) solution. (a) Time-lapse photos of the cortisol assay progressing in the microchannels at different times (0.5, 1.0, 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5 min), (b) Still photograph after completion of the reaction at 6.5 min. Colour intensity variability in the 15 channels was found to be 2% (%RSD). (c) Graph showing the colour intensity change at different times.
