Figure 2.

Inhibition of growth by piperine in lung adenocarcinoma (A549), breast adenocarcinoma (MDA-MB-231) and hepatocellular carcinoma (HepG2) cells. (A) HepG2, (B) MDA-MB-231 and (C) A549 cells were treated or not (CTR) with 20, 40, 80, 160 and 320 μM piperine for 72 h, and the cell viability was monitored by MTT assay at 570 nm using a Beckman Coulter AD 340 plate-reading spectrophotometer. The following formula has been used to calculate the percentage of viable cells: (viable cells) % = (OD of drug-treated sample/OD of untreated sample) × 100. Data are representative of three independent experiments ± SD. * p ≤ 0.05 vs. untreated cells (CTR).