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. 2020 Apr 8;7(4):19. doi: 10.3390/medicines7040019

Figure 7.

Figure 7

Effect of piperine on TGF-β receptors (TGFβRI and TGFβRII) and ERK 1/2 and SMAD2 activation in A549 cells treated with TGF-β1. A549 cells were plated in serum free medium and pretreated with 20, 40 and 80 μM piperine for 24 h. After treatment, cells were or not stimulated with 1 ng/mL recombinant human TGFβ1. The cells were then collected, washed with PBS and lysed in RIPA buffer. Western blot analysis was employed to evaluate (A) the expression of TGFβRI and TGFβRII receptors, (B) phospho-SMAD2 and (C) phospho-p44/42 MAPK (ERK1/2).