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Protein biotinylation catalyzed by biotin protein ligase (BPL). Biotin ligates with ATP to produce the reaction intermediate, biotinyl-5′-AMP. Biotinyl-5′-AMP is employed to covalently attach the biotinyl moiety onto a specific lysine residue present in the biotin-accepting protein substrate. The chemical structure of biotinyl-acylsulfamide adenosine (BASA), a chemical analog of the reaction intermediate, is shown.
