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. 2020 Apr 7;8(4):80. doi: 10.3390/biomedicines8040080

Figure 4.

Figure 4

Epitopic profiles of the wild-type NS5A of HCV 1a, 1b, and 3a predicting localization of CD8+ T cell epitopes in the Russian (a) and “global” (c) sequences, and CD4+ T cell epitopes in Russian (e) and “global” (g) sequences; Difference of the epitopic scores of HCV 1a, 1b and 3a with and without RAS in RAS-harboring region between aa 20 to 100 for CD8+ T cell epitopes in Russian (b) and “global” (d) sequences, and CD4+ T cell epitopes in Russian (f) and “global” (h) sequences, positions of RAS are highlighted on curves in diamonds. Predictions were done using NetMHCpan-4.0 and NetMHCIIpan-3.2 and were based on a set of globally prevalent HLA-A, HLA-B, HLA-C and HLA-DR molecules extracted from the Allele Frequency Net Database and 5 HLA-DP and 6 HLA-DQ molecules. Epitope profiles were constructed for each HCV subtype by for each protein position calculating the number of predicted HLA binding peptides overlapping that position weighed by the frequency of the given HLA and normalized so that the max value is 1 in each run of analysis.