Figure 3.

Electron microscopic images of the preparation of natural bovine α-crystallin from the eye lens nucleus: (A) preparation field; (B) a selection of single particles of the most representative population of the α-crystallin complex (asymmetric, bean-shaped particles with dimensions of about 15 × 20 nm). This population is represented by particles of a larger size compared to that of particles isolated from the cortex. Preparation conditions for EM analysis: C = 0.2 mg/mL, incubation for 30 min at 37 °C in 20 mM Tris-HCl buffer (pH 7.5), 100 mM NaCl, and 1 mM EDTA.