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. 2020 Apr 9;8(4):84. doi: 10.3390/biomedicines8040084

Figure 2.

Figure 2

Figure 2

Cotreatment of EGCG and TRAIL increased the number of terminal deoxynucleotidyl transferase-dT-mediated dUTP nick end labelling (TUNEL)-positive cells and induced poly (adenosine diphosphate-ribose) polymerase (PARP) cleavage and caspase 8 activation in SW480 and HCT116 cells. (A) Effect of EGCG and TRAIL on the number of TUNEL-positive cells in SW480 and HCT116 cells. SW480 and HCT116 cells were treated with TRAIL 25 ng/mL and/or EGCG 40 µM for TUNEL staining. The fluorescent signals from fragmented DNA (green), and DAPI (blue) were visualized and photographed by FLUOVIEW FV10i confocal microscopy. Magnification bar = 50 µm. Bar graphs represent quantification of TUNEL-positive cells (%). (B) Effect of TRAIL and EGCG on PARP cleavage and caspase 8 in in SW480 and HCT116 cells. SW480 and HCT116 cells were treated with TRAIL (25 ng/mL) and/or EGCG (20, 40 μM) for 24 h. Cell lysates were prepared and subjected to Western blotting with the indicated antibodies. β-actin was used as an internal control.