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. 2020 Apr 24;10(4):48. doi: 10.3390/life10040048

Figure 1.

Figure 1

Maternal obesity is associated with upregulation of Satb2 mRNA in the mediobasal hypothalamus of female offspring at GD17.5. (A) Image shows GD17.5 mediobasal hypothalamic region, with the dashed line indicating dissected region containing the arcuate nucleus (Arc) and ventromedial nucleus (VMN) for qPCR analysis. Scale bar is 500 μm. VMN, ventromedial nucleus of the hypothalamus; DMH, dorsomedial hypothalamic nucleus; Arc, arcuate nucleus; 3V, third ventricle. (B) Bar graph depicts qPCR analysis of Satb2 mRNA expression in the mediobasal hypothalamus of control and mHFD offspring at GD17.5. Data are normalized to two validated reference genes and expressed relative to control males as mean fold change ± SEM from n = 3–4 independent pregnancies per treatment group, with p ≤ 0.05 *.