Table 1.

Primer sequences and the thermal cycles for amplification reactions of the ITS, matK, rbcL, trnH-psbA regions.

Barcode	Primer Name	Primer Sequence	Thermal Cycle	Source
ITS	ITS1F	5′CTTGGTCATTTAGAGGAAGTAA3′	Denaturing: 94 °C/30 sec Annealing: 55 °C/40 sec Extending: 72 °C/1 min	[17,18]
	ITS4R	5′TCCTCCGCTTATTGATATGC3′
matK	390F	5′CGATCTATTCATTCAATATTTC3′	Denaturing: 94 °C/1 min Annealing: 48 °C/30 sec Extending: 72 °C/1 min	[6,19]
	1326R	5′TCTAGCACACGAAAGTCGAAGT3′
rbcL	aF	5′ATGTCACCACAAACAGAGACTAAAGC3′	Denaturing: 94 °C/30 sec Annealing: 55 °C/1 min Extending: 70 °C/1 min	[20]
	aR	5′CTTCTGCTACAAATAAGAATCGATCTCTCCA3′
trnH-psbA	trnHF_05	5′CGCGCATGGTGGATTCACAATCC3′	Denaturing: 95 °C/30 sec Annealing: 5 °C/20 sec Extending: 72 °C/20 sec	[21]
	psbA3′f	5′GTTATGCATGAACGTAATGCTC3′