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. 2020 Apr 4;9(4):71. doi: 10.3390/biology9040071

Figure 1.

Figure 1

Construction and characterization of an NB cell line with an introduced frameshift in MGAT2. The MGAT2 gene was silenced in the HuNB cell line using CRISPR/Cas9 technology. Coding sequences of the MGAT2 gene from 1 to 42 is shown for the parental and N-glycosylation mutant cell lines (A). The mutant cell line has a c inserted following the 22nd nucleotide, which is denoted in bold purple font. The purple font reveals the different codons in the sequence caused by the frameshift mutation. Typical flow cytometry plots of fluorescently labelled L-PHA (left panels), E-PHA (left middle panels), and GNL (right middle panels) bound to HuNB (top panels) and HuNB(-MGAT2) (bottom panels) cell lines are shown (B). Mean fluorescence intensity values of all three lectins bound to each of the cell lines were obtained from 4 experiments (C). * p < 0.01.