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. 2020 Apr 4;9(4):71. doi: 10.3390/biology9040071

Figure 8.

Figure 8

MMP-2 protein levels are lowered with increased hybrid and oligomannose types of N-glycans in NB cell lines. Conditioned medium was harvested from confluent plates. MMP-2 was detected by zymography (right panels), and western blots (middle panels) of the human and rat NB cell lines (A,B). Coomassie blue-stained gel (left panels) indicates that similar amounts of protein were present in the conditioned medium (A,B). Western blot of β-tubulin (right panels) and coomassie blue-stained gel (left panels) of whole cell lysates indicate that similar amounts of cells were used to generate MMP-2 in conditioned medium. (C,D). The reduction in activity and immunoband intensities between HuNB and HuNB(-MGAT2) were 29% ± 4% (n = 7) and 37% ± 11% (n = 4) respectively, and also, between ratNB and ratNB(-Mgat2), 40% ± 8% (n = 6) and 62% ±8% (n = 3), respectively. The n value denotes number of bands from three separate experiments for both human and rat. Horizontal lines adjacent to blots and gels indicate molecular weight standards in kDa: 250, 150, 100, 75, 50, 37, and 25, from top to bottom. Top marker was unobserved for western blot in panel C. Black and grey arrows point to MMP-2 and β-tubulin, respectively.