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. Author manuscript; available in PMC: 2020 May 19.
Published in final edited form as: J Vis Exp. 2019 Aug 30;(150):10.3791/60024. doi: 10.3791/60024

Figure 1: Selective intra-bronchial HCl instillation produces unilateral lung injury defined by alveolar barrier breach and neutrophil infiltration.

Figure 1:

(A) Representation of the cannulation of the murine left mainstem bronchus for selective instillation of HCl into the left lung. (B) Resected right (RL) and left (LL) lungs exposed to selective acid instillation and perfused following intravenous Evan’s blue dye. (C) Quantification of interstitial Evan’s blue dye from homogenized, perfused lung 24 h after acid injury or sham control; figure adapted from Abdulnour et al. 20146. Values represent mean ± SEM, where n ≥ 5. *p < 0.05, Mann-Whitney U Test. (D) Representative flow cytometry of intravascular (I.V.; fluorophore 1) and interstitial (I.S.; fluorophore 2) neutrophils as percent of total CD45+ cells in processed lung 24 h after acid injury.