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. 2020 Mar 19;11(5):615–623. doi: 10.1021/acsmedchemlett.9b00664

Figure 1.

Figure 1

Upper panel: binding isotherms for MST signals versus peptide concentrations. SOCS3 derived peptides were used with a serial dilution (1:1) by preparing 14–16 samples on average of the following stock solutions: ESSCONG 404 μM, KIRCONG chim 409 μM, restKIRESSCONG 408 μM in labeling buffer at pH 7.5. Lower panel: overlay of CD spectra in TFE/H20 0–75% v/v. Spectra were acquired in 10 mM phosphate buffer at pH 7.4, at 25 °C, in mixtures with TFE (2,2,2-trifluoroethanol) and peptides concentration was 50 μM.