Real-time PCR analysis
of STAT-dependent genes in macrophages.
RAW264.7 cells were stimulated with IFNγ+IL-6 for 1 h in the
absence or presence of 12.5 μM of SOCS3 peptidomimetics and
NC peptide. The mRNA expression levels of Cxcl10
(A), Ccl5 (B), and Nox2 (C) were
normalized to 18S and expressed as fold increases over basal condition
(arbitrary set as 1). Mean ± SEM of three independent experiments
(#P < 0.05, ##P < 0.01, ###P < 0.001 vs basal; *P < 0.05, **P < 0.01, ***P < 0.001 vs cytokines).