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. 2020 May 14;15:3415–3431. doi: 10.2147/IJN.S230949

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© 2020 Yuan et al.

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Effects of La₂O₃ NPs and MPs on testicular cells apoptosis. TUNEL and immunohistochemical staining of BAX (A-B) was detected at × 400 magnification. Apoptotic testicular cells in the control group (a), NL group (b), NM group (c), NH group (d) and WM group (e). Arrows indicated the positive cells, and histograms showing the percentages of TUNEL and BAX-positive cells (f). Exposure of mice to La₂O₃ NPs and MPs down-regulated of Bcl-2, and up-regulated both in the gene and protein levels of BAX in NH group compared with control group (Figure 7C-D) (P<0.05). The results of Bcl-2 and BAX protein expression in mouse testicular tissue were determined by Western blotting. β-Actin was used as the internal control (Da-b). *P< 0.05 vs control.