Table 1.
List of Primers Used for Real-Time RT-PCR
| Function | Gene Name | Primer Sequence (5ʹ-3ʹ) | Product (bp) | Genbank Accession |
|---|---|---|---|---|
| Testosterone synthesis-related genes | StAR | F: CGGGTGGATGGGTCAAGTTC R: GCACTTCGTCCCCGTTCTC |
188 | NM_011485.5 |
| CYP11A1 | F: AGGTCCTTCAATGAGATCCCTT R: TCCCTGTAAATGGGGCCATAC |
137 | NM_019779.4 | |
| CYP17A1 | F: AGTCAAAGACACCTAATGCCAAG R: ACGTCTGGGGAGAAACGGT |
83 | NM_007809.3 | |
| LHR | F: CTCGCCCGACTATCTCTCAC R: ACGACCTCATTAAGTCCCCTG |
77 | NM_013582.3 | |
| Nrf-2/ARE pathway-related genes | Keap-1 | F: CGGGGACGCAGTGATGTATG R: TGTGTAGCTGAAGGTTCGGTTA |
85 | NM_016679.4 |
| Nrf-2 | F: TAGATGACCATGAGTCGCTTGC R: GCCAAACTTGCTCCATGTCC |
153 | NM_010902.4 | |
| NQO1 | F: AGGATGGGAGGTACTCGAATC R: TGCTAGAGATGACTCGGAAGG |
127 | NM_008706.5 | |
| HO-1 | F: AGGTACACATCCAAGCCGAGA R: CATCACCAGCTTAAAGCCTTCT |
86 | NM_010442.2 | |
| GSH-Px | F: GAAGTGCGAAGTGAATGG R: TGTCGATGGTACGAAAGC |
224 | NM_008160.6 | |
| Inflammation | iNOS | F: GTTCTCAGCCCAACAATACAAGA R: GTGGACGGGTCGATGTCAC |
127 | NM_010927.4 |
| IL-1β | F: GAAATGCCACCTTTTGACAGTG R: TGGATGCTCTCATCAGGACAG |
116 | NM_008361.4 | |
| TNF-α | F: CATGGATCTCAAAGACAACCAA R: CTCCTGGTATGAAATGGCAAAT |
193 | NM_013693.3 | |
| COX-2 | F: TACAACAACTCCATCCTCCTTG R: TTCATCTCTCTGCTCTGGTCAA |
186 | NM_011198.4 | |
| Apoptosis | Bcl-2 | F: GGTCTTCAGAGACAGCCAGG R: GATCCAGGATAACGGAGGCT |
113 | NM_177410.3 |
| BAX | F: GATCAGCTCGGGCACTTTAG R: TTGCTGATGGCAACTTCAAC |
120 | NM_007527.3 | |
| Reference | β-actin | F: GTGCTATGTTGCTCTAGACTTCG R: ATGCCACAGGATTCCATACC |
174 | NM_007393.5 |