Figure 4.
2354glu alleviates lipid accumulation in acute alcoholic hepatosteatosis. C57BL/6 mice were gavaged with ethanol (5 g·kg−1) every 12 hr for a total of three doses to induce acute hepatic steatosis. 2354glu (100 and 50 mg·kg−1) was intraperitoneally injected simultaneously with ethanol administration. (a) Chemical structure of 2354glu. (b) Animal experimental procedure. (c) HE staining, Oil red O staining (200× original magnification). (d) Positive area of Oil Red O staining was analysed with Image Pro‐Plus 6.0. All histograms represent the mean ± SD of six independent assays with at least three replicates. # P < .05, significantly different from normal group; *P < .05, significantly different from ethanol group