Figure 5.

2354glu regulates LKB1–AMPK–SREBP1 in acute alcoholic hepatosteatosis. (a) Total and phosphorylated AMPKα and LKB1 protein levels in mouse livers were determined using western blot analysis. (b) Each immunoreactive band of phosphorylated and total protein of AMPK or LKB1 was normalized against GAPDH. Then the ratio of phosphorylated to total protein of AMPK or LKB1 was calculated. (c) Each immunoreactive band was normalized against GAPDH and relative to normal to control for unwanted sources of variation. (d) Immunohistochemistry for SREBP1 (200× original magnification). (e) Relative mRNA expression of SREBP1 targeting genes‐Fasn, Acly, and PPARα targeting genes Cpt2 and Abcd2. All histograms represent the mean ± SD of six independent assays with at least three replicates. ^# P < .05, significantly different from normal group; *P < .05, significantly different from ethanol group