Figure 3.

Functional expression profile of somatosensory TRP channels in human stem cell‐derived sensory neurons (hSCDS) neurons. (a) Changes in intracellular calcium in single hSCDS neurons stimulated with the TRP channel agonists menthol (100 μM), MO (100 μM), capsaicin (Caps;1 μM), and pregnenolone sulphate (PS;40 μM) and with a high K⁺ solution to probe excitability. (b) Venn diagram showing the pattern of responses to TRP channel agonists in hSCDS neurons (n = 1,180 cells in five independent differentiations). (c) Intracellular calcium measurements showing reversible inhibition of PS‐evoked responses by isosakuranetin (5 μM). PS‐responsive hSCDS neurons also responded to the synthetic TRPM3 agonist CIM0216 (1 μM). (d) Quantification of calcium responses for experiments as in panel (c) (n = 72). Isosa, isosakuranetin; MO, mustard oil [Colour figure can be viewed at http://wileyonlinelibrary.com]