Figure 3.

CRAMP deficiency causes NLRP3 inflammasome over‐activation and increased apoptosis in renal I/R‐induced injury. (a) Cell apoptosis was estimated by TUNEL staining. The percentage of apoptosis was measured relative to the photographic area, scale bar: 50 μm. (b) Western blot and quantitative analysis of Bax, Bcl‐2, and cleaved caspase‐3 in kidney tissues. GAPDH was used as loading control (n = 8 per group). (c) Western blot and quantitative analysis of NLRP3, cleaved caspase‐1 p20, cleaved IL‐1β, and cleaved IL‐18 expression in kidney tissues. GAPDH was used as loading control (n = 8 per group). TUNEL, terminal deoxynucleotidyl transferase‐mediated digoxigenin‐deoxyuridine nick‐end labelling. The results are shown as mean ± SEM. *P < .05, significantly different as indicated; one‐way ANOVA followed by Tukey's test [Colour figure can be viewed at http://wileyonlinelibrary.com]