Fig. 3.

Baicalein is a PR antagonist in T47D cells without reducing T47D cell proliferation. a HRE/Luciferase activity of baicalein alone. b HRE/Luciferase activity of baicalein in combination with 100 nM P4. c Representative western blot for PR-B and PR-A levels in T47D cells treated with 100 nM P4 or baicalein for 24 h. d Densitometry of total PR levels in T47D cells after P4 or baicalein treatment. e qPCR of TRIM22 in T47D cells after treatment with P4 (100 nM), RU486 (1 μM) and baicalein (10 μM) as indicated. f Proliferation of T47D cells after treatment with P4 or baicalein. g Proliferation of T47D cells after treatment with E2 and P4 as indicated. h Proliferation of T47D cells after treatment with E2 and baicalein as indicated. N≥3. Data represent mean ± SEM. Significantly different from 0 μM or Control. *P < 0.05; **P < 0.01; ***P < 0.001