Fig. 4.

Baicalein is a glucocorticoid agonist in OVCAR5. a–c Molecular docking of tangeretin, wogonin, and baicalein with GR, crystal structure PDB ID:3e7c. a Top-ranked docking pose for tangeretin with GR, showing side chains of GR residues within 3.5 Å of tangeretin. GR residues include Cys736, Gln642, Phe623, Gln570, and Asn564. Potential hydrogen bond interactions are shown as dashed lines. b Top-ranked docking pose for wogonin with GR, showing side chains of GR residues within 4.0 Å of wogonin. GR residues include Cys736, Gln642, Phe623, and Asn564. c Top-ranked docking pose for baicalein with GR, showing side chains of GR residues within 3.5 Å of baicalein. GR residues include Thr780, Cys736, Tyr735, Gln642, Phe623, Leu563, and Phe749. Potential hydrogen bond interactions are shown as dashed lines. d–i HRE/Luc of tangeretin (d, e), wogonin (f, g), and baicalein (h, i) in OVCAR 5 cells alone or in combination with 100 nM dexamethasone. N ≥3. Data represent mean ± SEM. Significantly different from 0 μM or control. *P < 0.05; **P < 0.01; ***P < 0.001. Bars without common letter are significantly different ^a-bP < 0.05