Fig. 2.

ABCA8 suppresses HCC cell proliferation and tumorigenesis in vitro and in vivo. a Western blot analysis of the transfection efficiency of ABCA8 in cell lines. b Proliferation rate was analyzed by a CCK-8 assay of indicated HCC cells. c Representative images of colony formation assays are shown on the left; the number of foci was counted as shown on the right. d and e Silencing expression of ABCA8 decreased Huh7 cell subcutaneous and orthotopic xenograft growth in nude mice, whereas ABCA8 overexpression had the opposite effect. Tumor volume and weight are shown on the right (n = 6/group). f Immunohistochemical detection of Ki-67 protein levels in xenograft tissues, Scale bars: 100× = 100 μm. Data are means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001