Table 1.

Summary of testing methodologies and parasite strains described in this report

Murine (in vivo) models	Strains	Parasite name	Origin	Assay description	References
CQ-sensitive	NK 173	Plasmodium berghei	Not reported	Not reported
	ANKA	P. berghei	Not reported	Not reported	[38–40]
		Plasmodium vinckei petteri	Not reported	Not reported
In vitro models
CQ-sensitive	3D7	P. falciparum	Not reported	Parasite lactate dehydrogenase (pLDH) assay	[41, 42]
			Not reported	Parasite growth inhibition assay	[43]
			Not reported	Translation inhibitory assay	[44]
	D6	P. falciparum	Sierra Leone	Incorporated G-3H hypoxanthine assay	[41, 42, 45, 46]
				Parasite lactate dehydrogenase (pLDH) assay	[47, 48]
				Non-radioactive Malaria SYBR Green I assay	[49, 50]
				Modified non-radioactive Malaria SYBR Green I assay	[42, 49, 51]
	D10	P. falciparum	Not reported	pLDH assay	[42, 52]
	F32	P. falciparum	Tanzania	Not reported
	FCA20	P. falciparum	Ghana	Not reported
	K1	P. falciparum	Thailand	Modified [3H]-hypoxanthine incorporation assay and [3H]-hypoxanthine incorporation assay	[53–56]
	NF54	P. falciparum	Not reported	[3H]-hypoxanthine incorporation assay	[53–56]
CQ-resistant	Dd2	P. falciparum	Not reported	Non-radioactive Malaria SYBR Green I assay	[49, 50]
	FcM29	P. falciparum	Cameroon	Not reported
	FcB1	P. falciparum	Colombia	[3H]-hypoxanthine incorporation assay	[41]
	K1	P. falciparum	Thailand	Modified [3H]-hypoxanthine incorporation assay and [3H]-hypoxanthine incorporation assay	[53–56]
	W2	P. falciparum	Indochina	Modified non-radioactive Malaria SYBR Green I assay	[42, 49, 51]
				Incorporated G-3H hypoxanthine assay	[45, 46]
				Non-radioactive Malaria SYBR Green I assay	[49, 50]
	NF54	P. falciparum		[3H]-hypoxanthine incorporation assay	[41]
CQ- and pyrimethamine-resistant	K1	P. falciparum	Thailand	Modified [3H]-hypoxanthine incorporation assay and [3H]-hypoxanthine incorporation assay	[53–55]
	NF54	P. falciparum	Thailand	[3H]-hypoxanthine incorporation assay	[54]
Multidrug-resistant	Dd2	P. falciparum	Not reported	[3H]-hypoxanthine incorporation assay	[53–56]
	K1	P. falciparum	Thailand	Parasite lactate dehydrogenase (pLDH) assay	[52, 57]
	NF54	P. falciparum	Not reported	[3H]-hypoxanthine incorporation assay	[53–56]
	W2	P. falciparum	Indochina	[3H]-hypoxanthine incorporation assay	[45, 46]
	W2mef	P. falciparum	Not reported	[3H]-hypoxanthine incorporation assay	[53–56]